Nanogel receptors for high isoelectric point protein detection: influence of electrostatic and covalent polymer-protein interactions.
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ABSTRACT:
SUBMITTER: Wechsler ME
PROVIDER: S-EPMC7377432 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Publications
Nanogel receptors for high isoelectric point protein detection: influence of electrostatic and covalent polymer-protein interactions.
Wechsler Marissa E ME Dang H K H Jocelyn HKHJ Dahlhauser Samuel D SD Simmonds Susana P SP Reuther James F JF Wyse Jordyn M JM VandeWalle Abigail N AN Anslyn Eric V EV Peppas Nicholas A NA
Chemical communications (Cambridge, England) 20200504 45
An aldehyde acrylate-based functional monomer was incorporated into poly(N-isopropylacrylamide-co-methacrylic acid) nanogels for use as protein receptors. The aldehyde component forms dynamic imines with surface exposed lysine residues, while carboxylic acid/carboxylate moieties form electrostatic interactions with high isoelectric point proteins. Together, these interactions effect protein adsorption and recognition. ...[more]
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