Dataset Information

Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance.

ABSTRACT:

Background

Biofunctionalization of titanium surfaces can improve host responses, especially considering the time for osteointegration and patient recovery. This prompted us to modify titanium surfaces with alendronate and albumin and to investigate the behavior of osteoblasts on these surfaces.

Methods

The biofunctionalization of titanium surfaces was characterized using classical physicochemical approaches and later used to challenge pre-osteoblast cells up to 24 h. Then their viability and molecular behavior were investigated using mitochondrial dehydrogenase activity and RTq-PCR technologies, respectively. Potential stimulus of extracellular remodeling was also investigated by zymography.

Results

Our data indicates a differential behavior of cells responding to the surfaces, considering the activity of mitochondrial dehydrogenases. Molecularly, the differential expression of genes related with cell adhesion highlighted the importance of Integrin-β1, Fak, and Src. These 3 genes were significantly decreased in response to titanium surfaces modified with alendronate, but this behavior was reverted when alendronate was associated with albumin. Alendronate-modified surfaces promoted a significant increase on ECM remodeling, as well as culminating with greater gene activity related to the osteogenic phenotype (Runx2, Alp, Bsp).

Conclusion

Altogether, our study found interesting osteogenic behavior of cells in response to alendronate and albumin surfaces, which indicates the need for in vivo analyses to better consider these surfaces before clinical trials within the biomedical field.

SUBMITTER: Albano CS

PROVIDER: S-EPMC7378701 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Publications

Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance.

Albano Carolina Simão CS Moreira Gomes Anderson A da Silva Feltran Geórgia G da Costa Fernandes Célio Junior CJ Trino Luciana Daniele LD Zambuzzi Willian Fernando WF Lisboa-Filho Paulo Noronha PN

Heliyon 20200721 7

<h4>Background</h4>Biofunctionalization of titanium surfaces can improve host responses, especially considering the time for osteointegration and patient recovery. This prompted us to modify titanium surfaces with alendronate and albumin and to investigate the behavior of osteoblasts on these surfaces.<h4>Methods</h4>The biofunctionalization of titanium surfaces was characterized using classical physicochemical approaches and later used to challenge pre-osteoblast cells up to 24 h. Then their vi ...[more]

PMID: 32715131

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Dataset Information

Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance.

Background

Methods

Results

Conclusion

Publications

Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance.

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