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Glycated hemoglobin concentrations of red blood cells minimally increase during storage under standard blood banking conditions.

ABSTRACT: BACKGROUND:Few and inconsistent data exist describing the effect of storage duration on glycated hemoglobin (HbA1c) concentrations of red blood cells (RBCs), impeding interpretation of HbA1c values in transfused diabetic patients. Hence the aim of this study was to evaluate to what extent HbA1c concentrations of RBCs change during the maximum allowed storage period of 42 days. STUDY DESIGN AND METHODS:Blood was drawn from 16 volunteers, leukofiltered, and stored under standard blood banking conditions. HbA1c concentrations of RBCs were measured on Days 1 and 42 of storage using three different validated devices (ion-exchange high-performance liquid chromatography Method A1 and A2, turbidimetric immunoassay Method B). RESULTS:Mean HbA1c concentrations of RBCs on Day 1 were 5.3 ± 0.3% (Method A1), 5.4 ± 0.4% (Method A2), and 5.1 ± 0.4% (Method B). HbA1c concentrations increased to 5.6 ± 0.3% (A1, p < 0.0001), 5.7 ± 0.3% (A2, p = 0.004), and 5.5 ± 0.4% (B, p < 0.0001) on Day 42, respectively, corresponding to a 1.06-fold increase across all methods. Glucose concentrations in the storage solution of RBCs decreased from 495 ± 27 to 225 ± 55 mg/dL (p < 0.0001), confirming that stored RBCs were metabolically active. CONCLUSION:These results suggest a significant, albeit minor, and most likely clinically insignificant increase in HbA1c concentrations during storage of RBCs for 42 days.

SUBMITTER: Prosenz J

PROVIDER: S-EPMC7379282 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Glycated hemoglobin concentrations of red blood cells minimally increase during storage under standard blood banking conditions.

Prosenz Julian J Öhlinger Thomas T Müllner Ernst W EW Marculescu Rodrig R Gerner Christopher C Salzer Ulrich U Kiefer Florian W FW Baron David M DM

Transfusion 20181001 2

<h4>Background</h4>Few and inconsistent data exist describing the effect of storage duration on glycated hemoglobin (HbA1c) concentrations of red blood cells (RBCs), impeding interpretation of HbA1c values in transfused diabetic patients. Hence the aim of this study was to evaluate to what extent HbA1c concentrations of RBCs change during the maximum allowed storage period of 42 days.<h4>Study design and methods</h4>Blood was drawn from 16 volunteers, leukofiltered, and stored under standard blo ...[more]

PMID: 30727042

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Project description:Abnormally shaped red blood cells (RBCs), called poikilocytes, can cause anemia. At present, the biochemical abnormalities in poikilocytes are not well understood. Normal RBCs and poikilocytes were analyzed using whole-blood and single-cell methods. Poikilocytes were induced in rat blood by intragastrically administering titanium dioxide (TiO2) nanoparticles. Complete blood count and inductively coupled plasma mass spectrometry analyses were performed on whole-blood to measure average RBC morphology, blood hemoglobin (HGB), iron content, and other blood parameters. Follow-up confocal Raman spectroscopy was performed on single RBCs to analyze cell-type-specific HGB content. Two types of poikilocytes, acanthocytes and echinocytes, were observed in TiO2 blood samples, along with normal RBCs. Acanthocytes (diameter 7.7??±??0.5???m) and echinocytes (7.6??±??0.6???m) were microscopically larger (p??<??0.05) than normal RBCs (6.6??±??0.4???m) found in control blood samples (no TiO2 administration). Similarly, mean corpuscular volume was higher (p??<??0.05) in TiO2 whole-blood (70.70??±??1.97??fl) than in control whole-blood (67.42??±??2.03??fl). Poikilocytes also had higher HGB content. Mean corpuscular hemoglobin was higher (p??<??0.05) in TiO2 whole-blood (21.84??±??0.75??pg) than in control whole-blood (20.8??±??0.32??pg). Iron content was higher (p??<??0.001) in TiO2 whole-blood (697.0??±??24.5??mg??/??l) than in control whole-blood (503.4??±??38.5??mg??/??l), which supports elevated HGB as iron is found in HGB. HGB-associated Raman bands at 1637, 1585, and 1372??cm??-??1 had higher (p??<??0.001) amplitudes in acanthocytes and echinocytes than in RBCs from control blood and normal RBCs from TiO2 blood. Further, the 1585-cm??-??1 band had a lower (p??< ?0.05) amplitude in normal RBCs from TiO2 versus control RBCs. This represents biochemical abnormalities in normal appearing RBCs. Overall, poikilocytes, especially acanthocytes, have elevated HGB.

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Glycated hemoglobin concentrations of red blood cells minimally increase during storage under standard blood banking conditions.

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Glycated hemoglobin concentrations of red blood cells minimally increase during storage under standard blood banking conditions.

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