Ontology highlight
ABSTRACT:
SUBMITTER: Juszkiewicz S
PROVIDER: S-EPMC7381030 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Juszkiewicz Szymon S Slodkowicz Greg G Lin Zhewang Z Freire-Pritchett Paula P Peak-Chew Sew-Yeu SY Hegde Ramanujan S RS
eLife 20200713
Translation of aberrant mRNAs can cause ribosomes to stall, leading to collisions with trailing ribosomes. Collided ribosomes are specifically recognised by ZNF598 to initiate protein and mRNA quality control pathways. Here we found using quantitative proteomics of collided ribosomes that EDF1 is a ZNF598-independent sensor of ribosome collisions. EDF1 stabilises GIGYF2 at collisions to inhibit translation initiation in cis via 4EHP. The GIGYF2 axis acts independently of the ZNF598 axis, but eac ...[more]