[Effects of stachyine on apoptosis in an Aβ_25-35-induced PC12 cell model of Alzheimer's disease].

ABSTRACT:

Objective

To investigate the effects of stachydrine (STA) on apoptosis of Aβ_25-35-induced PC12 cells mimicking Alzheimer's disease and explore the mechanisms.

Methods

The differential genes of STA were analyzed based on GSE85871 data, and the target genes of STA were identified using STITCH database. PC12 cells were treated with Aβ_25-35 to establish a cell model of Alzheimer's disease, and the changes in cell viability and cell cycle in response to STA treatment were assessed using MTT assay and flow cytometry, respectively. RT-PCR and Western blotting were used to detect the relevant gene or protein expressions in the treated cells.

Results

GSE85871 data showed 37 up-regulated genes and 48 down-regulated genes in cells following treatment with S

SUBMITTER: Qu L 

PROVIDER: S-EPMC7386225 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature