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Topoisomerase III-? is required for efficient replication of positive-sense RNA viruses.

ABSTRACT: Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-? (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target.

SUBMITTER: Prasanth KR

PROVIDER: S-EPMC7386308 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Topoisomerase III-β is required for efficient replication of positive-sense RNA viruses.

Prasanth K Reddisiva KR Hirano Minato M Fagg W Samuel WS McAnarney Eileen T ET Shan Chao C Xie Xuping X Hage Adam A Pietzsch Colette A CA Bukreyev Alexander A Rajsbaum Ricardo R Shi Pei-Yong PY Bedford Mark T MT Bradrick Shelton S SS Menachery Vineet V Garcia-Blanco Mariano A MA

Antiviral research 20200728

Based on genome-scale loss-of-function screens we discovered that Topoisomerase III-β (TOP3B), a human topoisomerase that acts on DNA and RNA, is required for yellow fever virus and dengue virus-2 replication. Remarkably, we found that TOP3B is required for efficient replication of all positive-sense-single stranded RNA viruses tested, including SARS-CoV-2. While there are no drugs that specifically inhibit this topoisomerase, we posit that TOP3B is an attractive anti-viral target. ...[more]

PMID: 32735900

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