Dataset Information

Dysbiosis in intestinal microbiome linked to fecal blood determined by direct hybridization.

ABSTRACT: The important physiological and pathophysiological roles of intestinal human microbiome (HMB) in human health have been emerging, owing to the access to molecular biology techniques. Herein we evaluated, for the first time, the intestinal HMB through direct hybridization approach using n-counter flex DX technology which bypasses the amplification procedure currently applied by other technologies to study the human microbiome. To this purpose, a clinical study was carried out on fecal samples, recruiting both healthy volunteers (N-FOB) and subjects positive for occult blood (P-FOB). A relevant custom panel of 79 16S rRNA target gene was engineered and 32 of them displayed a variation between the two clusters of subjects. Our findings revealed that bacteria belonging to Proteobacteria have higher distribution in P-FOB describing dysbiosis. Similarly, Bacteroidetes and Firmicutes phylum display high distribution in P-FOB. Of interest, the presence of Clostridium difficile that belongs to Firmicutes phylum displayed about 70% of low presence in N-FOB compared to P-FOB subjects. Only one bacterium belonging to the Actinobacteria phylum, the Bifidobacterium bifidum, was present.

SUBMITTER: Cafiero C

PROVIDER: S-EPMC7387388 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Dysbiosis in intestinal microbiome linked to fecal blood determined by direct hybridization.

Cafiero Concetta C Re Agnese A Pisconti Salvatore S Trombetti Marina M Perri Mariarita M Colosimo Manuela M D'Amato Gerardo G Gallelli Luca L Cannataro Roberto R Molinario Clelia C Fazio Alessia A Caroleo Maria Cristina MC Cione Erika E

3 Biotech 20200728 8

The important physiological and pathophysiological roles of intestinal human microbiome (HMB) in human health have been emerging, owing to the access to molecular biology techniques. Herein we evaluated, for the first time, the intestinal HMB through direct hybridization approach using n-counter flex DX technology which bypasses the amplification procedure currently applied by other technologies to study the human microbiome. To this purpose, a clinical study was carried out on fecal samples, re ...[more]

PMID: 32821643

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Dataset Information

Dysbiosis in intestinal microbiome linked to fecal blood determined by direct hybridization.

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Dysbiosis in intestinal microbiome linked to fecal blood determined by direct hybridization.

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