Ontology highlight
ABSTRACT:
SUBMITTER: Marston DJ
PROVIDER: S-EPMC7388658 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Marston Daniel J DJ Vilela Marco M Huh Jaewon J Ren Jinqi J Azoitei Mihai L ML Glekas George G Danuser Gaudenz G Sondek John J Hahn Klaus M KM
Nature chemical biology 20200518 8
Here we generate fluorescence resonance energy transfer biosensors for guanine exchange factors (GEFs) by inserting a fluorescent protein pair in a structural 'hinge' common to many GEFs. Fluorescent biosensors can map the activation of signaling molecules in space and time, but it has not been possible to quantify how different activation events affect one another or contribute to a specific cell behavior. By imaging the GEF biosensors in the same cells as red-shifted biosensors of Rho GTPases, ...[more]