OvCa-Chip microsystem recreates vascular endothelium-mediated platelet extravasation in ovarian cancer.

ABSTRACT: In ovarian cancer, platelet extravasation into the tumor and resulting metastasis is thought to be regulated mostly by the vascular endothelium. Because it is difficult to dissect complex underlying events in murine models, organ-on-a-chip methodology is applied to model vascular and platelet functions in ovarian cancer. This system (OvCa-Chip) consists of microfluidic chambers that are lined by human ovarian tumor cells interfaced with a 3-dimensional endothelialized lumen. Subsequent perfusion with human platelets within the device's vascular endothelial compartment under microvascular shear conditions for 5 days uncovered organ-to-molecular-level contributions of the endothelium to triggering platelet extravasation into tumors. Further, analysis of effluents available from the device's

SUBMITTER: Saha B 

PROVIDER: S-EPMC7391144 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature