Ontology highlight
ABSTRACT:
SUBMITTER: Sheets MB
PROVIDER: S-EPMC7393974 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
ACS synthetic biology 20200121 2
Optogenetic tools can provide direct and programmable control of gene expression. Light-inducible recombinases, in particular, offer a powerful method for achieving precise spatiotemporal control of DNA modification. However, to-date this technology has been largely limited to eukaryotic systems. Here, we develop optogenetic recombinases for <i>Escherichia coli</i> that activate in response to blue light. Our approach uses a split recombinase coupled with photodimers, where blue light brings the ...[more]