Unknown

Dataset Information

0

Distinct subnetworks of the thalamic reticular nucleus.


ABSTRACT: The thalamic reticular nucleus (TRN), the major source of thalamic inhibition, regulates thalamocortical interactions that are critical for sensory processing, attention and cognition1-5. TRN dysfunction has been linked to sensory abnormality, attention deficit and sleep disturbance across multiple neurodevelopmental disorders6-9. However, little is known about the organizational principles that underlie its divergent functions. Here we performed an integrative study linking single-cell molecular and electrophysiological features of the mouse TRN to connectivity and systems-level function. We found that cellular heterogeneity in the TRN is characterized by a transcriptomic gradient of two negatively correlated gene-expression profiles, each containing hundreds of genes. Neurons in the extremes of this transcriptomic gradient express mutually exclusive markers, exhibit core or shell-like anatomical structure and have distinct electrophysiological properties. The two TRN subpopulations make differential connections with the functionally distinct first-order and higher-order thalamic nuclei to form molecularly defined TRN-thalamus subnetworks. Selective perturbation of the two subnetworks in vivo revealed their differential role in regulating sleep. In sum, our study provides a comprehensive atlas of TRN neurons at single-cell resolution and links molecularly defined subnetworks to the functional organization of thalamocortical circuits.

SUBMITTER: Li Y 

PROVIDER: S-EPMC7394718 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications


The thalamic reticular nucleus (TRN), the major source of thalamic inhibition, regulates thalamocortical interactions that are critical for sensory processing, attention and cognition<sup>1-5</sup>. TRN dysfunction has been linked to sensory abnormality, attention deficit and sleep disturbance across multiple neurodevelopmental disorders<sup>6-9</sup>. However, little is known about the organizational principles that underlie its divergent functions. Here we performed an integrative study linkin  ...[more]

Similar Datasets

| S-EPMC4205482 | biostudies-other
2020-07-22 | GSE145273 | GEO
| S-EPMC4169194 | biostudies-literature
2023-04-24 | GSE210720 | GEO
2023-03-31 | GSE226333 | GEO
| S-EPMC8324895 | biostudies-literature
| S-EPMC4281280 | biostudies-literature
| S-EPMC6789150 | biostudies-literature
| S-EPMC8068728 | biostudies-literature
| S-EPMC3864346 | biostudies-literature