Rare osteosarcoma cell subpopulation protein array and profiling using imaging mass cytometry and bioinformatics analysis.
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ABSTRACT:
SUBMITTER: Batth IS
PROVIDER: S-EPMC7395380 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Publications
Rare osteosarcoma cell subpopulation protein array and profiling using imaging mass cytometry and bioinformatics analysis.
Batth Izhar S IS Meng Qing Q Wang Qi Q Torres Keila E KE Burks Jared J Wang Jing J Gorlick Richard R Li Shulin S
BMC cancer 20200731 1
<h4>Background</h4>Single rare cell characterization represents a new scientific front in personalized therapy. Imaging mass cytometry (IMC) may be able to address all these questions by combining the power of MS-CyTOF and microscopy.<h4>Methods</h4>We have investigated this IMC method using < 100 to up to 1000 cells from human sarcoma tumor cell lines by incorporating bioinformatics-based t-Distributed Stochastic Neighbor Embedding (t-SNE) analysis of highly multiplexed IMC imaging data. We tes ...[more]
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