Dataset Information

Development of a Multilocus Sequence Typing Scheme for Giardia intestinalis.

ABSTRACT: Giardia intestinalis is an intestinal protozoan most commonly found in humans. It has been grouped into 8 assemblages (A-H). Markers such as the glutamate dehydrogenase gene, triose phosphate isomerase and beta-giardin (β-giardin) have been widely used for genotyping. In addition, different genetic targets have been proposed as a valuable alternative to assess diversity and genetics of this microorganism. Thus, our objective was to evaluate new markers for the study of the diversity and intra-taxa genetic structure of G. intestinalis in silico and in DNA obtained from stool samples. We analysed nine constitutive genes in 80 complete genome sequences and in a group of 24 stool samples from Colombia. Allelic diversity was evaluated by locus and for the concatenated sequence of nine loci that could discriminate up to 53 alleles. Phylogenetic reconstructions allowed us to identify AI, AII and B assemblages. We found evidence of intra- and inter-assemblage recombination events. Population structure analysis showed genetic differentiation among the assemblages analysed.

SUBMITTER: Higuera A

PROVIDER: S-EPMC7397270 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Development of a Multilocus Sequence Typing Scheme for <i>Giardia intestinalis</i>.

Higuera Adriana A Muñoz Marina M López Myriam Consuelo MC Reyes Patricia P Urbano Plutarco P Villalobos Oswaldo O Ramírez Juan David JD

Genes 20200708 7

<i>Giardia intestinalis</i> is an intestinal protozoan most commonly found in humans. It has been grouped into 8 assemblages (A-H). Markers such as the glutamate dehydrogenase gene, triose phosphate isomerase and beta-giardin (β-giardin) have been widely used for genotyping. In addition, different genetic targets have been proposed as a valuable alternative to assess diversity and genetics of this microorganism. Thus, our objective was to evaluate new markers for the study of the diversity and i ...[more]

PMID: 32650382

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Dataset Information

Development of a Multilocus Sequence Typing Scheme for Giardia intestinalis.

Publications

Development of a Multilocus Sequence Typing Scheme for <i>Giardia intestinalis</i>.

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