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Vibration synergistically enhances IL-1? and TNF-? in compressed human periodontal ligament cells in the frequency-dependent manner.

ABSTRACT: Objectives:To investigate whether mechanical vibration at 30 or 60 Hz combined with compressive force alter IL-1? and TNF-? expression in human periodontal ligament (hPDL) cells. Methods:hPDL cells isolated from the roots of first premolar teeth extracted from four independent donors were cultured and exposed to vibration (0.3 g, 20 min per cycle, every 24 h for 3 cycles) at 30 or 60 Hz (V30 or V60), 2.0 g/cm2 compressive force for 2 days (CF), or a combination of compressive force and vibration at 30 Hz or 60 Hz (V30CF or V60CF). Quantitative real-time polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assays (ELISAs) were used to determine IL-1? and TNF-? mRNA and protein, respectively. Results:The levels of IL-1? and TNF-? did not alter in groups V30 and V60. While, they were upregulated in groups CF, V30CF and V60CF. In addition, IL-1? mRNA and TNF-? mRNA and protein were expressed at significantly higher levels in group V30CF compared to CF group. However, IL-1? protein levels between V30CF and CF groups did not reach statistical significance. Conclusions:30 Hz vibration had the synergistic effects with compressive force on the upregulation of IL-1? mRNA and TNF-? mRNA and protein in PDL cells, while 60 Hz vibration did not have this synergistic effect.

SUBMITTER: Benjakul S

PROVIDER: S-EPMC7397387 | biostudies-literature | 2020 Oct-Dec

REPOSITORIES: biostudies-literature

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Vibration synergistically enhances IL-1β and TNF-α in compressed human periodontal ligament cells in the frequency-dependent manner.

Benjakul Sutiwa S Unat Boontarika B Thammanichanon Peungchaleoy P Leethanakul Chidchanok C

Journal of oral biology and craniofacial research 20200622 4

<h4>Objectives</h4>To investigate whether mechanical vibration at 30 or 60 Hz combined with compressive force alter IL-1β and TNF-α expression in human periodontal ligament (hPDL) cells.<h4>Methods</h4>hPDL cells isolated from the roots of first premolar teeth extracted from four independent donors were cultured and exposed to vibration (0.3 g, 20 min per cycle, every 24 h for 3 cycles) at 30 or 60 Hz (V30 or V60), 2.0 g/cm<sup>2</sup> compressive force for 2 days (CF), or a combination of compr ...[more]

PMID: 32775184

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Project description:The structural and functional integrity of bone-periodontal ligament (PDL)-cementum complex stems from the load-bearing attachment sites (entheses) between soft (PDL) and hard (bone, cementum) tissues. These attachment sites are responsible for the maintenance of a bone-PDL-cementum complex biomechanical function. The objective was to investigate changes in spatiotemporal expression of key biomolecules in developing and functionally active entheses.Multilabeling technique was performed on hemimandibles of 3 wk and 3 mo-old scleraxis-GFP transgenic mice for CD146, CD31, NG2, osterix and bone sialoprotein. Regions of dominant stretch within the PDL were evaluated by identifying directionality of collagen fibrils, PDL fibroblasts and PDL cell cytoskeleton.CD146+ cells adjacent to CD31+ vasculature were identified at PDL-bone enthesis. NG2+ cells were located at coronal bone-PDL and apical cementum-PDL entheses in the 3-wk-old group, but at 3 mo, NG2 was positive at the entheses of the apical region and alveolar crest. NG2 and osterix were colocalized at the osteoid and cementoid regions of the PDL-bone and PDL-cementum entheses. Bone sialoprotein was prominent at the apical region of 3-wk-old mice. The directionality of collagen fibers, fibroblasts and their cytoskeleton overlapped, except in the apical region of 3 wk.Colocalization of biomolecules at zones of the PDL adjacent to attachment sites may be essential for the formation of precementum and osteoid interfaces at a load-bearing bone-PDL-tooth fibrous joint. Biophysical cues resulting from development and function can regulate recruitment and differentiation of stem cells potentially from a vascular origin toward osteo- and cemento-blastic lineages at the PDL-bone and PDL-cementum entheses. Investigating the coupled effect of biophysical and biochemical stimuli leading to cell differentiation at the functional attachment sites is critical for developing regeneration strategies to enable functional reconstruction of the periodontal complex.

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Dataset Information

Vibration synergistically enhances IL-1? and TNF-? in compressed human periodontal ligament cells in the frequency-dependent manner.

Publications

Vibration synergistically enhances IL-1β and TNF-α in compressed human periodontal ligament cells in the frequency-dependent manner.

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