Ontology highlight
ABSTRACT:
SUBMITTER: Lu B
PROVIDER: S-EPMC7402673 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Lu Bo B Dong Liting L Yi Danyang D Zhang Meiling M Zhu Chenxu C Li Xiaoyu X Yi Chengqi C
eLife 20200723
Tn5-mediated transposition of double-strand DNA has been widely utilized in various high-throughput sequencing applications. Here, we report that the Tn5 transposase is also capable of direct tagmentation of RNA/DNA hybrids in vitro. As a proof-of-concept application, we utilized this activity to replace the traditional library construction procedure of RNA sequencing, which contains many laborious and time-consuming processes. Results of <u>T</u>ransposase-assisted <u>R</u>NA/DN<u>A</u> hybrids ...[more]