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Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques.

ABSTRACT: In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2.

SUBMITTER: Carossino M

PROVIDER: S-EPMC7406679 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques.

Carossino Mariano M Ip Hon S HS Richt Juergen A JA Shultz Kendra K Harper Kimberly K Loynachan Alan T AT Del Piero Fabio F Balasuriya Udeni B R UBR

Archives of virology 20200805 10

In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope®-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2. ...[more]

PMID: 32761270

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