Detection of SARS-CoV-2 by RNAscope® in situ hybridization and immunohistochemistry techniques.
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ABSTRACT:
SUBMITTER: Carossino M
PROVIDER: S-EPMC7406679 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Publications
Detection of SARS-CoV-2 by RNAscope<sup>®</sup> in situ hybridization and immunohistochemistry techniques.
Carossino Mariano M Ip Hon S HS Richt Juergen A JA Shultz Kendra K Harper Kimberly K Loynachan Alan T AT Del Piero Fabio F Balasuriya Udeni B R UBR
Archives of virology 20200805 10
In situ hybridization (ISH) and immunohistochemistry (IHC) are essential tools to characterize SARS-CoV-2 infection and tropism in naturally and experimentally infected animals and also for diagnostic purposes. Here, we describe three RNAscope<sup>®</sup>-based ISH assays targeting the ORF1ab, spike, and nucleocapsid genes and IHC assays targeting the spike and nucleocapsid proteins of SARS-CoV-2. ...[more]
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