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Antioxidant and Moisturizing Properties of Carboxymethyl Chitosan with Different Molecular Weights.

ABSTRACT: This research aimed to synthesize carboxymethyl chitosan (CMCH) from different molecular weights of chitosan including low MW (L, 50-190 kDa), medium MW (M, 210-300 kDa) and high MW (H, 310-375 kDa) on the antioxidant and moisturizing properties. The L-CMCH, M-CMCH and H-CMCH improved the water solubility by about 96%, 90% and 89%, respectively when compared to native chitosan. Higher MW resulted in more viscous of CMCH. For antioxidant properties, IC50 values of DPPH and ABTS radical scavenging activity for L-CMCH were 1.70 and 1.37 mg/mL, respectively. The L-CMCH had higher antioxidant properties by DPPH and ABTS radical scavenging assay and FRAP. The moisturizing properties on pig skin using a Corneometer® showed that 0.5% H-CMCH significantly presented (p ? 0.05) greater moisturizing effect than that of untreated-skin, distilled water, propylene glycol and pure chitosan from three molecular weights.

SUBMITTER: Chaiwong N

PROVIDER: S-EPMC7407185 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Antioxidant and Moisturizing Properties of Carboxymethyl Chitosan with Different Molecular Weights.

Chaiwong Nareekan N Leelapornpisid Pimporn P Jantanasakulwong Kittisak K Rachtanapun Pornchai P Seesuriyachan Phisit P Sakdatorn Vinyoo V Leksawasdi Noppol N Phimolsiripol Yuthana Y

Polymers 20200628 7

This research aimed to synthesize carboxymethyl chitosan (CMCH) from different molecular weights of chitosan including low MW (L, 50-190 kDa), medium MW (M, 210-300 kDa) and high MW (H, 310-375 kDa) on the antioxidant and moisturizing properties. The L-CMCH, M-CMCH and H-CMCH improved the water solubility by about 96%, 90% and 89%, respectively when compared to native chitosan. Higher MW resulted in more viscous of CMCH. For antioxidant properties, IC<sub>50</sub> values of DPPH and ABTS radical ...[more]

PMID: 32605198

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Project description:The interaction between bovine antithrombin, a plasma proteinase inhibitor, and heparin species of different molecular weights was studied. A commercial heparin preparation was divided by gel chromatography into a number of fractions with average molecular weights ranging from 6000 to 34700. Each of these fractions was further fractionated by affinity chromatography on matrix-bound antithrombin. In the latter procedure, those heparin fractions that had molecular weights lower than about 14000 were separated into three peaks. The material in the first of these was not adsorbed on the column, and the other two peaks corresponded to the low-affinity and high-affinity peaks described previously. In contrast, high-molecular-weight heparin samples gave only the low-affinity and high-affinity fractions. U.v. difference absorption studies showed that the non-adsorbed heparin fraction bound to antithrombin in solution with a binding constant at physiological ionic strength only slightly lower than that of low-affinity heparin. The division between the two fractions thus is arbitrary and only dependent on the conditions selected for the affinity-chromatography experiment. Stoicheiometries and binding constants for the binding of several high-affinity heparin species to antithrombin were determined by fluorescence titrations. High-affinity heparin fractions of equal elution positions in the beginning of the peaks of the affinity chromatographies, but with different molecular weights, showed stoicheiometries that were not experimentally distinguishable from 1:1 and also had no appreciable differences in binding constants. However, the anticoagulant activities, calculated on a molar basis, of these fractions increased markedly with molecular weight, a behaviour that thus cannot be explained by differences in the binding of the fractions to antithrombin. In contrast, high-affinity samples of similar molecular weights, which were eluted at increasing ionic strengths from matrix-linked antithrombin, were found to have an increasing proportion of chains with two binding sites for antithrombin and also to have progressively higher binding constants. These binding properties at least partly explain the increasing anticoagulant activities that were observed for these fractions.

Dataset Information

Antioxidant and Moisturizing Properties of Carboxymethyl Chitosan with Different Molecular Weights.

Publications

Antioxidant and Moisturizing Properties of Carboxymethyl Chitosan with Different Molecular Weights.

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