Homocysteine-induced electrical remodeling via the mediation of IP₃R1/Nav1.5 signaling pathway.

ABSTRACT: Inositol-1,4,5-triphosphate-receptor 1 (IP₃R1), a Ca²⁺ channel in the sarcoplasmic reticulum membrane, is an effective regulator of Ca²⁺ release involved in the pathology of most cardiovascular diseases. Our study aim to investigate the underlying mechanism by which IP₃R1 signaling mediates the process of homocysteine (Hcy)-induced Ca²⁺ accumulation via interaction with sodium current (Nav1.5) in atrium. We utilized whole-cell patch-clamp analysis and flow cytometry to detect the abnormal electrical activity in mouse atrial myocytes (MACs) obtained from C57B6 mice fed with high-Hcy diet. The results represented not only an increase in protein levels of Nav1.5 and IP₃R1, but also an enhanced intracellular levels of Ca^2+<

SUBMITTER: Han L 

PROVIDER: S-EPMC7407712 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature