Unknown

Dataset Information

0

Vitamin D Prevents Pancreatic Cancer-Induced Apoptosis Signaling of Inflammatory Cells.


ABSTRACT: Combined approaches based on immunotherapy and drugs supporting immune effector cell function might increase treatment options for pancreatic ductal adenocarcinoma (PDAC), vitamin D being a suitable drug candidate. In this study, we evaluated whether treatment with the vitamin D analogue, calcipotriol, counterbalances PDAC induced and SMAD4-associated intracellular calcium [Ca2+]i alterations, cytokines release, immune effector function, and the intracellular signaling of peripheral blood mononuclear cells (PBMCs). Calcipotriol counteracted the [Ca2+]i depletion of PBMCs induced by SMAD4-expressing PDAC cells, which conditioned media augmented the number of calcium flows while reducing whole [Ca2+]i. While calcipotriol inhibited spontaneous and PDAC-induced tumor necrosis factor alpha (TNF-?) release by PBMC and reduced intracellular transforming growth factor beta (TGF-?), it did not counteract the lymphocytes proliferation induced in allogenic co-culture by PDAC-conditioned PBMCs. Calcipotriol mainly antagonized PDAC-induced apoptosis and partially restored PDAC-inhibited NF-?B signaling pathway. In conclusion, alterations induced by PDAC cells in the [Ca2+]i of immune cells can be partially reverted by calcipotriol treatment, which promotes inflammation and antagonizes PBMCs apoptosis. These effects, together with the dampening of intracellular TGF-?, might result in an overall anti-tumor effect, thus supporting the administration of vitamin D in PDAC patients.

SUBMITTER: Moz S 

PROVIDER: S-EPMC7408286 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

Vitamin D Prevents Pancreatic Cancer-Induced Apoptosis Signaling of Inflammatory Cells.

Moz Stefania S   Contran Nicole N   Facco Monica M   Trimarco Valentina V   Plebani Mario M   Basso Daniela D  

Biomolecules 20200715 7


Combined approaches based on immunotherapy and drugs supporting immune effector cell function might increase treatment options for pancreatic ductal adenocarcinoma (PDAC), vitamin D being a suitable drug candidate. In this study, we evaluated whether treatment with the vitamin D analogue, calcipotriol, counterbalances PDAC induced and SMAD4-associated intracellular calcium [Ca<sup>2+</sup>]<sub>i</sub> alterations, cytokines release, immune effector function, and the intracellular signaling of p  ...[more]

Similar Datasets

| S-EPMC2500132 | biostudies-literature
| S-EPMC4576995 | biostudies-literature
| S-EPMC3734826 | biostudies-other
| S-EPMC3286294 | biostudies-literature
| S-EPMC2893235 | biostudies-literature
| S-EPMC3392497 | biostudies-literature
| S-EPMC4144808 | biostudies-literature
| S-EPMC3400624 | biostudies-literature
| S-EPMC3907072 | biostudies-literature
| S-EPMC3890699 | biostudies-literature