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Upregulating ?-hexosaminidase activity in rodents prevents ?-synuclein lipid associations and protects dopaminergic neurons from ?-synuclein-mediated neurotoxicity.

ABSTRACT: Sandhoff disease (SD) is a lysosomal storage disease, caused by loss of ?-hexosaminidase (HEX) activity resulting in the accumulation of ganglioside GM2. There are shared features between SD and Parkinson's disease (PD). ?-synuclein (aSYN) inclusions, the diagnostic hallmark sign of PD, are frequently found in the brain in SD patients and HEX knockout mice, and HEX activity is reduced in the substantia nigra in PD. In this study, we biochemically demonstrate that HEX deficiency in mice causes formation of high-molecular weight (HMW) aSYN and ubiquitin in the brain. As expected from HEX enzymatic function requirements, overexpression in vivo of HEXA and B combined, but not either of the subunits expressed alone, increased HEX activity as evidenced by histochemical assays. Biochemically, such HEX gene expression resulted in increased conversion of GM2 to its breakdown product GM3. In a neurodegenerative model of overexpression of aSYN in rats, increasing HEX activity by AAV6 gene transfer in the substantia nigra reduced aSYN embedding in lipid compartments and rescued dopaminergic neurons from degeneration. Overall, these data are consistent with a paradigm shift where lipid abnormalities are central to or preceding protein changes typically associated with PD.

SUBMITTER: Brekk OR

PROVIDER: S-EPMC7409708 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Upregulating β-hexosaminidase activity in rodents prevents α-synuclein lipid associations and protects dopaminergic neurons from α-synuclein-mediated neurotoxicity.

Brekk Oeystein R OR Korecka Joanna A JA Crapart Cecile C CC Huebecker Mylene M MacBain Zachary K ZK Rosenthal Sara Ann SA Sena-Esteves Miguel M Priestman David A DA Platt Frances M FM Isacson Ole O Hallett Penelope J PJ

Acta neuropathologica communications 20200806 1

Sandhoff disease (SD) is a lysosomal storage disease, caused by loss of β-hexosaminidase (HEX) activity resulting in the accumulation of ganglioside GM2. There are shared features between SD and Parkinson's disease (PD). α-synuclein (aSYN) inclusions, the diagnostic hallmark sign of PD, are frequently found in the brain in SD patients and HEX knockout mice, and HEX activity is reduced in the substantia nigra in PD. In this study, we biochemically demonstrate that HEX deficiency in mice causes fo ...[more]

PMID: 32762772

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Dataset Information

Upregulating ?-hexosaminidase activity in rodents prevents ?-synuclein lipid associations and protects dopaminergic neurons from ?-synuclein-mediated neurotoxicity.

Publications

Upregulating β-hexosaminidase activity in rodents prevents α-synuclein lipid associations and protects dopaminergic neurons from α-synuclein-mediated neurotoxicity.

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