Unknown

Dataset Information

0

Global Analysis of Small Non-Coding RNA Populations across Tissues in the Malaria Vector, Anopheles gambiae.


ABSTRACT: Malaria is a major global health problem, where the anautogenous female mosquito Anopheles gambiae serves as a major vector. In order to combat this devastating disease, understanding mosquito physiology is paramount. Numerous studies in the vector field demonstrate that small non-coding RNAs (ncRNAs) play essential roles in numerous aspects of mosquito physiology. While our previous miRNA annotation work demonstrated expression dynamics across differing tissues, miRNAs represented less than 20% of all small ncRNAs in our small RNA-Seq libraries. To this end, we systematically classified multiple small ncRNA groups across mosquito tissues. Here we (i) determined a new enriched-midgut miRNA, (ii) updated the piRNA annotation in ovaries with a genomic map of unique-mapping piRNAs, (iii) identified pan-tissue and tissue-enriched mRNA-derived small ncRNAs, and (iv) assessed AGO1- and AGO2- loading of candidate small ncRNAs. Continued research will broaden our view of small ncRNAs and greatly aide in our understanding on how these molecules contribute to mosquito physiology.

SUBMITTER: Bryant WB 

PROVIDER: S-EPMC7411766 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Global Analysis of Small Non-Coding RNA Populations across Tissues in the Malaria Vector, <i>Anopheles gambiae</i>.

Bryant William Bart WB   Ray Savanna S   Mills Mary Katherine MK  

Insects 20200630 7


Malaria is a major global health problem, where the anautogenous female mosquito <i>Anopheles gambiae</i> serves as a major vector. In order to combat this devastating disease, understanding mosquito physiology is paramount. Numerous studies in the vector field demonstrate that small non-coding RNAs (ncRNAs) play essential roles in numerous aspects of mosquito physiology. While our previous miRNA annotation work demonstrated expression dynamics across differing tissues, miRNAs represented less t  ...[more]

Similar Datasets

| S-EPMC6505144 | biostudies-literature
| S-EPMC2840125 | biostudies-literature
| S-EPMC3113458 | biostudies-literature
| S-EPMC2500179 | biostudies-literature
| S-EPMC6026373 | biostudies-literature
| S-EPMC7462981 | biostudies-literature
| S-EPMC4043680 | biostudies-literature
| S-EPMC387373 | biostudies-literature
2010-12-31 | GSE19756 | GEO
2024-09-15 | GSE243215 | GEO