Dataset Information

Long-Distance Movement of Mineral Deficiency-Responsive mRNAs in Nicotiana Benthamiana/Tomato Heterografts.

ABSTRACT: Deficiencies in essential mineral nutrients such as nitrogen (N), phosphorus (P), and iron (Fe) severely limit plant growth and crop yield. It has been discovered that both the local sensing system in roots and shoot-to-root systemic signaling via the phloem are involved in the regulation of the adaptive alterations in roots, in response to mineral deficiency. mRNAs are one group of molecules with systemic signaling functions in response to intrinsic and environmental cues; however, the importance of shoot-to-root mobile mRNAs stimulated by low mineral levels is not fully understood. In this study, we established a Nicotiana benthamiana/tomato heterograft system to identify shoot-to-root mobile mRNAs that are produced in response to low N, P or Fe. Multiple long-distance mobile mRNAs were identified to be associated with low mineral levels and a few of them may play important roles in hormonal metabolism and root architecture alteration. A comparison of the mobile mRNAs from our study with those identified from previous studies showed that very few transcripts are conserved among different species.

SUBMITTER: Xia C

PROVIDER: S-EPMC7412313 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Long-Distance Movement of Mineral Deficiency-Responsive mRNAs in <i>Nicotiana Benthamiana</i>/Tomato Heterografts.

Xia Chao C Huang Jing J Lan Hai H Zhang Cankui C

Plants (Basel, Switzerland) 20200710 7

Deficiencies in essential mineral nutrients such as nitrogen (N), phosphorus (P), and iron (Fe) severely limit plant growth and crop yield. It has been discovered that both the local sensing system in roots and shoot-to-root systemic signaling via the phloem are involved in the regulation of the adaptive alterations in roots, in response to mineral deficiency. mRNAs are one group of molecules with systemic signaling functions in response to intrinsic and environmental cues; however, the importan ...[more]

PMID: 32664315

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Project description:Viroids are infectious non-coding RNAs that infect plants. During infection, viroid RNAs are targeted by Dicer-like proteins, generating viroid-derived small RNAs (vd-sRNAs) that can guide the sequence specific cleavage of cognate host mRNAs via an RNA silencing mechanism. To assess the involvement of these pathways in pathogenesis associated with nuclear-replicating viroids, high-throughput sequencing of sRNAs and degradome analysis were carried out on tomato and Nicotiana benthamiana plants infected by potato spindle tuber viroid (PSTVd). Both hosts develop similar stunting and leaf curling symptoms when infected by PSTVd, thus allowing comparative analyses. About one hundred tomato mRNAs potentially targeted for degradation by vd-sRNAs were initially identified. However, data from biological replicates and comparisons between mock and infected samples reduced the number of bona fide targets-i.e., those identified with high confidence in two infected biological replicates but not in the mock controls-to only eight mRNAs that encode proteins involved in development, transcription or defense. Somewhat surprisingly, results of RT-qPCR assays revealed that the accumulation of only four of these mRNAs was inhibited in the PSTVd-infected tomato. When these analyses were extended to mock inoculated and PSTVd-infected N. benthamiana plants, a completely different set of potential mRNA targets was identified. The failure to identify homologous mRNA(s) targeted by PSTVd-sRNA suggests that different pathways could be involved in the elicitation of similar symptoms in these two species. Moreover, no significant modifications in the accumulation of miRNAs and in the cleavage of their targeted mRNAs were detected in the infected tomato plants with respect to the mock controls. Taken together, these data suggest that stunting and leaf curling symptoms induced by PSTVd are elicited by a complex plant response involving multiple mechanisms, with RNA silencing being only one of the possible components.

Dataset Information

Long-Distance Movement of Mineral Deficiency-Responsive mRNAs in Nicotiana Benthamiana/Tomato Heterografts.

Publications

Long-Distance Movement of Mineral Deficiency-Responsive mRNAs in <i>Nicotiana Benthamiana</i>/Tomato Heterografts.

Similar Datasets

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