Dataset Information

Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5.

ABSTRACT: Pseudomonas fluorescens Pf-5 is a plant commensal bacterium that inhabits the rhizosphere and produces secondary metabolites that suppress soilborne plant pathogens. The complete sequence of the 7.1-Mb Pf-5 genome was determined. We analyzed repeat sequences to identify genomic islands that, together with other approaches, suggested P. fluorescens Pf-5's recent lateral acquisitions include six secondary metabolite gene clusters, seven phage regions and a mobile genomic island. We identified various features that contribute to its commensal lifestyle on plants, including broad catabolic and transport capabilities for utilizing plant-derived compounds, the apparent ability to use a diversity of iron siderophores, detoxification systems to protect from oxidative stress, and the lack of a type III secretion system and toxins found in related pathogens. In addition to six known secondary metabolites produced by P. fluorescens Pf-5, three novel secondary metabolite biosynthesis gene clusters were also identified that may contribute to the biocontrol properties of P. fluorescens Pf-5.

SUBMITTER: Paulsen IT

PROVIDER: S-EPMC7416659 | biostudies-literature | 2005 Jul

REPOSITORIES: biostudies-literature

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Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5.

Paulsen Ian T IT Press Caroline M CM Ravel Jacques J Kobayashi Donald Y DY Myers Garry S A GS Mavrodi Dmitri V DV DeBoy Robert T RT Seshadri Rekha R Ren Qinghu Q Madupu Ramana R Dodson Robert J RJ Durkin A Scott AS Brinkac Lauren M LM Daugherty Sean C SC Sullivan Stephen A SA Rosovitz Mary J MJ Gwinn Michelle L ML Zhou Liwei L Schneider Davd J DJ Cartinhour Samuel W SW Nelson William C WC Weidman Janice J Watkins Kisha K Tran Kevin K Khouri Hoda H Pierson Elizabeth A EA Pierson Leland S LS Thomashow Linda S LS Loper Joyce E JE

Nature biotechnology 20050626 7

Pseudomonas fluorescens Pf-5 is a plant commensal bacterium that inhabits the rhizosphere and produces secondary metabolites that suppress soilborne plant pathogens. The complete sequence of the 7.1-Mb Pf-5 genome was determined. We analyzed repeat sequences to identify genomic islands that, together with other approaches, suggested P. fluorescens Pf-5's recent lateral acquisitions include six secondary metabolite gene clusters, seven phage regions and a mobile genomic island. We identified vari ...[more]

PMID: 15980861

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Project description:BACKGROUND: Pseudomonas fluorescens Pf-5 is a plant-associated bacterium that inhabits the rhizosphere of a wide variety of plant species and and produces secondary metabolites suppressive of fungal and oomycete plant pathogens. The Pf-5 genome is rich in features consistent with its commensal lifestyle, and its sequence has revealed attributes associated with the strain's ability to compete and survive in the dynamic and microbiologically complex rhizosphere habitat. In this study, we analyzed mobile genetic elements of the Pf-5 genome in an effort to identify determinants that might contribute to Pf-5's ability to adapt to changing environmental conditions and/or colonize new ecological niches. RESULTS: Sequence analyses revealed that the genome of Pf-5 is devoid of transposons and IS elements and that mobile genetic elements (MGEs) are represented by prophages and genomic islands that collectively span over 260 kb. The prophages include an F-pyocin-like prophage 01, a chimeric prophage 03, a lambdoid prophage 06, and decaying prophages 02, 04 and 05 with reduced size and/or complexity. The genomic islands are represented by a 115-kb integrative conjugative element (ICE) PFGI-1, which shares plasmid replication, recombination, and conjugative transfer genes with those from ICEs found in other Pseudomonas spp., and PFGI-2, which resembles a portion of pathogenicity islands in the genomes of the plant pathogens Pseudomonas syringae and P. viridiflava. Almost all of the MGEs in the Pf-5 genome are associated with phage-like integrase genes and are integrated into tRNA genes. CONCLUSION: Comparative analyses reveal that MGEs found in Pf-5 are subject to extensive recombination and have evolved in part via exchange of genetic material with other Pseudomonas spp. having commensal or pathogenic relationships with plants and animals. Although prophages and genomic islands from Pf-5 exhibit similarity to MGEs found in other Pseudomonas spp., they also carry a number of putative niche-specific genes that could affect the survival of P. fluorescens Pf-5 in natural habitats. Most notable are an approximately 35-kb segment of "cargo" genes in genomic island PFGI-1 and bacteriocin genes associated with prophages 1 and 4.

Dataset Information

Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5.

Publications

Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5.

Similar Datasets

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