Ontology highlight
ABSTRACT:
SUBMITTER: Israeli O
PROVIDER: S-EPMC7416699 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 20200810
The genetic identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is based on viral RNA extraction prior to RT-qPCR assay. However, recent studies have supported the elimination of the extraction step. This study was performed to assess the necessity for the RNA extraction, by comparing the efficacy of RT-qPCR in several direct approaches versus the gold standard RNA extraction, in the detection of SARS-CoV-2 in laboratory samples, as well as in clinical oro-nasopharynge ...[more]