Isolation of Microglia from Mouse or Human Tissue.
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ABSTRACT:
SUBMITTER: Bordt EA
PROVIDER: S-EPMC7416840 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Publications
Isolation of Microglia from Mouse or Human Tissue.
Bordt Evan A EA Block Carina L CL Petrozziello Tiziana T Sadri-Vakili Ghazaleh G Smith Caroline J CJ Edlow Andrea G AG Bilbo Staci D SD
STAR protocols 20200603 1
Microglia are the innate immune cells of the central nervous system. Although numerous methods have been developed to isolate microglia from the brain, the method of dissociation and isolation can have a profound effect on the function of these highly dynamic cells. Here, we present an optimized protocol to isolate CD11b+ cells (microglia) from mouse or human brain tissue using magnetic bead columns. Isolated microglia can be used to model diseases with neuroinflammatory components for potential ...[more]
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