Dataset Information

Continuous Anaerobic Digestion of Wood Vinegar Wastewater From Pyrolysis: Microbial Diversity and Functional Genes Prediction.

ABSTRACT: Wood vinegar wastewater (WVWW) is the main by-product of biomass pyrolysis process, which is more suitable to use anaerobic digestion (AD) to achieve energy recovery due to its large amount of organic matter. In this study, the up-flow anaerobic sludge bed (UASB) reactor was used to investigate the continuous anaerobic transformation of WVWW with gradient concentrations (0.3, 0.675, 1, 2, 3, 4, 5, 6, and 7 g COD/L). Then, the changes of microbial community, diversity index and functional gene were analyzed in detail. The results revealed that WVWW showed good AD performance in continuous fermentation. WVWW of organic loading rate (OLR) of >8.58 g COD/L?d showed severe inhibition on biodegradability and methane production, which is mainly due to the toxic substances as compared with the control group. The bacterial communities were dominated by phyla of Chloroflexi, Firmicutes, Proteobacteria, Acidobacteria, Synergistetes, and Actinobacteria. The gene abundances related to energy production, carbohydrate transport and metabolism were relatively high, which are mainly responsible for carbon forms conversion and carbohydrate degradation. This study will provide a basis for the screening and enrichment of functional bacteria and genes.

SUBMITTER: Hua D

PROVIDER: S-EPMC7422680 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Continuous Anaerobic Digestion of Wood Vinegar Wastewater From Pyrolysis: Microbial Diversity and Functional Genes Prediction.

Hua Dongliang D Fan Qingwen Q Zhao Yuxiao Y Xu Haipeng H Chen Lei L Si Hongyu H Li Yan Y

Frontiers in bioengineering and biotechnology 20200805

Wood vinegar wastewater (WVWW) is the main by-product of biomass pyrolysis process, which is more suitable to use anaerobic digestion (AD) to achieve energy recovery due to its large amount of organic matter. In this study, the up-flow anaerobic sludge bed (UASB) reactor was used to investigate the continuous anaerobic transformation of WVWW with gradient concentrations (0.3, 0.675, 1, 2, 3, 4, 5, 6, and 7 g COD/L). Then, the changes of microbial community, diversity index and functional gene we ...[more]

PMID: 32850755

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Project description:We report, for the first time, extensive biologically mediated phosphate removal from wastewater during high-rate anaerobic digestion (AD). A hybrid sludge bed/fixed-film (packed pumice stone) reactor was employed for low-temperature (12°C) anaerobic treatment of synthetic sewage wastewater. Successful phosphate removal from the wastewater (up to 78% of influent phosphate) was observed, mediated by biofilms in the reactor. Scanning electron microscopy and energy dispersive X-ray analysis revealed the accumulation of elemental phosphorus (∼2%) within the sludge bed and fixed-film biofilms. 4', 6-diamidino-2-phenylindole (DAPI) staining indicated phosphorus accumulation was biological in nature and mediated through the formation of intracellular inorganic polyphosphate (polyP) granules within these biofilms. DAPI staining further indicated that polyP accumulation was rarely associated with free cells. Efficient and consistent chemical oxygen demand (COD) removal was recorded, throughout the 732-day trial, at applied organic loading rates between 0.4 and 1.5 kg COD m(-3) d(-1) and hydraulic retention times of 8-24 h, while phosphate removal efficiency ranged from 28 to 78% on average per phase. Analysis of protein hydrolysis kinetics and the methanogenic activity profiles of the biomass revealed the development, at 12°C, of active hydrolytic and methanogenic populations. Temporal microbial changes were monitored using Illumina MiSeq analysis of bacterial and archaeal 16S rRNA gene sequences. The dominant bacterial phyla present in the biomass at the conclusion of the trial were the Proteobacteria and Firmicutes and the dominant archaeal genus was Methanosaeta. Trichococcus and Flavobacterium populations, previously associated with low temperature protein degradation, developed in the reactor biomass. The presence of previously characterized polyphosphate accumulating organisms (PAOs) such as Rhodocyclus, Chromatiales, Actinobacter, and Acinetobacter was recorded at low numbers. However, it is unknown as yet if these were responsible for the luxury polyP uptake observed in this system. The possibility of efficient phosphate removal and recovery from wastewater during AD would represent a major advance in the scope for widespread application of anaerobic wastewater treatment technologies.

Dataset Information

Continuous Anaerobic Digestion of Wood Vinegar Wastewater From Pyrolysis: Microbial Diversity and Functional Genes Prediction.

Publications

Continuous Anaerobic Digestion of Wood Vinegar Wastewater From Pyrolysis: Microbial Diversity and Functional Genes Prediction.

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