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Influence of Lens Fluorescence on Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO) Fundus Imaging and Strategies for Its Compensation.

ABSTRACT: Purpose:To explore the contribution of crystalline lens fluorescence to fluorescence lifetimes measured with fluorescence lifetime imaging ophthalmoscopy (FLIO) and to propose a computational model to reduce the lens influence. Methods:FLIO, which detects autofluorescence decay over time in a short-wavelength spectral channel (SSC, 498-560 nm) and a long-wavelength spectral channel (LSC, 560-720 nm), was performed on 32 patients before and after cataract extraction. The mean autofluorescence lifetime (? m ) of the fundus was determined from a three-exponential fit of the postoperative fluorescence decays. The preoperative measurements were fit with series of exponential functions in which one fluorescence component was time-shifted in order to represent lens fluorescence. Results:Postoperatively, ? m was 185 ± 22 ps in the SSC and 209 ± 34 ps in the LSC at the posterior pole. These values were best reproduced by fitting the postoperative measurements with a three-exponential model with a time-shifted third fluorescence component (SSC, 203 ± 45 ps; LSC, 215 ± 29 ps), whereas disregarding time-shifted lens fluorescence resulted in significantly (P < 0.001) longer ? m values (SSC, 474 ± 206 ps; LSC, 215 ± 29 ps). The fluorescence of the cataract lens contributed to the total fluorescence by 54.2 ± 10.6% (SSC) and 29.5 ± 9.9% (LSC). Conclusions:Cataract lens fluorescence greatly alters fluorescence lifetimes measured at the fundus by FLIO, resulting in an overestimation of the lifetimes; however, this may be compensated for considerably by taking lens influence into account in the fitting model. Translational Relevance:This study investigates cataract fluorescence in FLIO and a mathematical model for compensation of this influence.

SUBMITTER: Brauer JL

PROVIDER: S-EPMC7422756 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Influence of Lens Fluorescence on Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO) Fundus Imaging and Strategies for Its Compensation.

Brauer Jakob Lauritz JL Schultz Rowena R Klemm Matthias M Hammer Martin M

Translational vision science & technology 20200709 8

<h4>Purpose</h4>To explore the contribution of crystalline lens fluorescence to fluorescence lifetimes measured with fluorescence lifetime imaging ophthalmoscopy (FLIO) and to propose a computational model to reduce the lens influence.<h4>Methods</h4>FLIO, which detects autofluorescence decay over time in a short-wavelength spectral channel (SSC, 498-560 nm) and a long-wavelength spectral channel (LSC, 560-720 nm), was performed on 32 patients before and after cataract extraction. The mean autof ...[more]

PMID: 32855860

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Project description:Fluorescence lifetime imaging ophthalmoscopy (FLIO) is a novel technique that measures in vivo autofluorescence intensity decay over time of endogenous fluorophores in the retina. The Heidelberg Engineering FLIO system was used to obtain two 30 degree scans centered on the fovea of both eyes. The FLIO system uses a 473nm blue scanning laser light source and the emitted fluorescence is detected in two wavelengths channels, short and long spectral channels (SSC, LSC). Since the mydriatic status influence the FLIO result, the impact of mydriasis on FLIO need to be clarified. In this prospective, observational study, the impact of mydriasis on measurements from fluorescence lifetime imaging ophthalmoscope (FLIO) images in normal subjects were evaluated. 12 healthy participants (24 eyes) were volunteered and all subjects were scanned twice and the mean fluorescence lifetime (?m) values were computed with dilation and without dilation on different days. Intraclass correlation coefficients (ICC) and coefficients of variation (CV) were calculated from the measured ?m in dilated, nondilated and between the dilated and non-dilated setting. Test duration was also compared and correlated with lifetimes in both settings. Repeatability was excellent for both the dilation and non-dilation settings (ICC; 0.967-0.996; 0.926-0.986, respectively). The agreement between the dilation and non-dilation settings, however, were lower (ICC; 0.688-0.970). The ?m in the non-dilation setting was significantly longer than in the dilation setting for the SSC (P<0.05). The FLIO test duration in the non-dilation setting was significantly longer than with dilation for the SSC (P <0.05). Although good repeatability in ?m measurements between imaging sessions were observed both with and without dilation, the agreement was not as good when comparing dilated with non-dilated measurements. Since FLIO without mydriasis results in longer ?m in the SSC and takes a longer time for image acquisition, maximal dilation is recommended for FLIO testing.

Dataset Information

Influence of Lens Fluorescence on Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO) Fundus Imaging and Strategies for Its Compensation.

Publications

Influence of Lens Fluorescence on Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO) Fundus Imaging and Strategies for Its Compensation.

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