Dataset Information

Analysis of the Relaxometric Properties of Extremely Rapidly Exchanging Gd³⁺ Chelates: Lessons from a Comparison of Four Isomeric Chelates.

ABSTRACT: Relaxometric analyses and in particular the use of fast-field cycling techniques have become routine in the study of paramagnetic metal complexes. The field dependence of the solvent proton relaxation properties (nuclear magnetic relaxation dispersion, NMRD) can provide unparalleled insights into the chemistry of these complexes. However, analyzing NMRD data is a multiparametric problem, and some sets of variables are mutually compensatory. Specifically, when fitting NMRD profiles, the metal-proton distance and the rotational correlation time constant have a push-pull relationship in which a change to one causes a predictable compensation in the other. A relaxometric analysis of four isomeric chelates highlights the pitfalls that await when fitting the NMRD profiles of chelates for which dissociative water exchange is extremely rapid. In the absence of independently verified values for one of these parameters, NMRD profiles can be fitted to multiple parameter sets. This means that NMRD fitting can inadvertently be used to buttress a preconceived notion of how the complex should behave when a different parameter set may more accurately describe the actual behavior. These findings explain why the effect of very rapid dissociative exchange on the hydration state of Gd³⁺ has remained obscured until only recently.

SUBMITTER: Webber BC

PROVIDER: S-EPMC7423567 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Analysis of the Relaxometric Properties of Extremely Rapidly Exchanging Gd<sup>3+</sup> Chelates: Lessons from a Comparison of Four Isomeric Chelates.

Webber Benjamin C BC Payne Katherine M KM Rust Lauren N LN Cassino Claudio C Carniato Fabio F McCormick Theresa T Botta Mauro M Woods Mark M

Inorganic chemistry 20200614 13

Relaxometric analyses and in particular the use of fast-field cycling techniques have become routine in the study of paramagnetic metal complexes. The field dependence of the solvent proton relaxation properties (nuclear magnetic relaxation dispersion, NMRD) can provide unparalleled insights into the chemistry of these complexes. However, analyzing NMRD data is a multiparametric problem, and some sets of variables are mutually compensatory. Specifically, when fitting NMRD profiles, the metal-pro ...[more]

PMID: 32536158

Similar Datasets

Project description:Recently, rational design of a new class of contrast agents (CAs), based on biopolymers (hydrogels), have received considerable attention in Magnetic Resonance Imaging (MRI) diagnostic field. Several strategies have been adopted to improve relaxivity without chemical modification of the commercial CAs, however, understanding the MRI enhancement mechanism remains a challenge. Methods: A multidisciplinary approach is used to highlight the basic principles ruling biopolymer-CA interactions in the perspective of their influence on the relaxometric properties of the CA. Changes in polymer conformation and thermodynamic interactions of CAs and polymers in aqueous solutions are detected by isothermal titration calorimetric (ITC) measurements and later, these interactions are investigated at the molecular level using NMR to better understand the involved phenomena. Water molecular dynamics of these systems is also studied using Differential Scanning Calorimetry (DSC). To observe relaxometric properties variations, we have monitored the MRI enhancement of the examined structures over all the experiments. The study of polymer-CA solutions reveals that thermodynamic interactions between biopolymers and CAs could be used to improve MRI Gd-based CA efficiency. High-Pressure Homogenization is used to obtain nanoparticles. Results: The effect of the hydration of the hydrogel structure on the relaxometric properties, called Hydrodenticity and its application to the nanomedicine field, is exploited. The explanation of this concept takes place through several key aspects underlying biopolymer-CA's interactions mediated by the water. In addition, Hydrodenticity is applied to develop Gadolinium-based polymer nanovectors with size around 200 nm with improved MRI relaxation time (10-times). Conclusions: The experimental results indicate that the entrapment of metal chelates in hydrogel nanostructures offers a versatile platform for developing different high performing CAs for disease diagnosis.

Dataset Information

Analysis of the Relaxometric Properties of Extremely Rapidly Exchanging Gd³⁺ Chelates: Lessons from a Comparison of Four Isomeric Chelates.

Publications

Analysis of the Relaxometric Properties of Extremely Rapidly Exchanging Gd<sup>3+</sup> Chelates: Lessons from a Comparison of Four Isomeric Chelates.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Analysis of the Relaxometric Properties of Extremely Rapidly Exchanging Gd3+ Chelates: Lessons from a Comparison of Four Isomeric Chelates.

Publications

Analysis of the Relaxometric Properties of Extremely Rapidly Exchanging Gd<sup>3+</sup> Chelates: Lessons from a Comparison of Four Isomeric Chelates.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Analysis of the Relaxometric Properties of Extremely Rapidly Exchanging Gd³⁺ Chelates: Lessons from a Comparison of Four Isomeric Chelates.