Ontology highlight
ABSTRACT:
SUBMITTER: Kim D
PROVIDER: S-EPMC7426837 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Kim Daesik D Lim Kayeong K Kim Da-Eun DE Kim Jin-Soo JS
Nature communications 20200813 1
Cpf1-linked base editors broaden the targeting scope of programmable cytidine deaminases by recognizing thymidine-rich protospacer-adjacent motifs (PAM) without inducing DNA double-strand breaks (DSBs). Here we present an unbiased in vitro method for identifying genome-wide off-target sites of Cpf1 base editors via whole genome sequencing. First, we treat human genomic DNA with dLbCpf1-BE ribonucleoprotein (RNP) complexes, which convert C-to-U at on-target and off-target sites and, then, with a ...[more]