Ontology highlight
ABSTRACT:
SUBMITTER: Wilkinson OJ
PROVIDER: S-EPMC7430649 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Wilkinson Oliver J OJ Carrasco Carolina C Aicart-Ramos Clara C Moreno-Herrero Fernando F Dillingham Mark S MS
Nucleic acids research 20200801 14
DNA2 is an essential enzyme involved in DNA replication and repair in eukaryotes. In a search for homologues of this protein, we identified and characterised Geobacillus stearothermophilus Bad, a bacterial DNA helicase-nuclease with similarity to human DNA2. We show that Bad contains an Fe-S cluster and identify four cysteine residues that are likely to co-ordinate the cluster by analogy to DNA2. The purified enzyme specifically recognises ss-dsDNA junctions and possesses ssDNA-dependent ATPase, ...[more]