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Revisiting the window of opportunity for cotranscriptional splicing in budding yeast.

ABSTRACT: We reported previously that, in budding yeast, transcription rate affects both the efficiency and fidelity of pre-mRNA splicing, especially of ribosomal protein transcripts. Here, we report that the majority of ribosomal protein transcripts with non-consensus 5' splice sites are spliced less efficiently when transcription is faster, and more efficiently with slower transcription. These results support the "window of opportunity" model, and we suggest a possible mechanism to explain these findings.

SUBMITTER: Aslanzadeh V

PROVIDER: S-EPMC7430680 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Revisiting the window of opportunity for cotranscriptional splicing in budding yeast.

Aslanzadeh Vahid V Beggs Jean D JD

RNA (New York, N.Y.) 20200521 9

We reported previously that, in budding yeast, transcription rate affects both the efficiency and fidelity of pre-mRNA splicing, especially of ribosomal protein transcripts. Here, we report that the majority of ribosomal protein transcripts with non-consensus 5' splice sites are spliced less efficiently when transcription is faster, and more efficiently with slower transcription. These results support the "window of opportunity" model, and we suggest a possible mechanism to explain these finding ...[more]

PMID: 32439718

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