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A mouse model of Staphylococcus aureus small intestinal infection.


ABSTRACT: Introduction. Staphylococcus aureus is a recognised cause of foodborne intoxication and antibiotic-associated diarrhoea (AAD), which are both mediated by staphylococcal enterotoxins. However, unlike foodborne intoxication, AAD appears to require infection of the host. While S. aureus intoxication is widely studied, little is known about S. aureus pathogenesis in the context of gastrointestinal infection.Aim. To develop a mouse model of S. aureus gastrointestinal infection.Methodology. An established AAD mouse model was adapted for S. aureus infection, and damage observed via histopathological analysis and immunostaining of intestinal tissues.Results. Various strains colonised the mouse model, and analysis showed that although clinical signs of disease were not seen, S. aureus infection induced damage in the small intestine, disrupting host structures essential for epithelial integrity. Studies using a staphylococcal enterotoxin B mutant showed that this toxin may contribute to damage during gastrointestinal infection.Conclusion. This work presents a new mouse model of S. aureus gastrointestinal infection, while also providing insight into the pathogenesis of S. aureus in the gut.

SUBMITTER: Larcombe S 

PROVIDER: S-EPMC7431102 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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A mouse model of <i>Staphylococcus aureus</i> small intestinal infection.

Larcombe Sarah S   Jiang Jhih-Hang JH   Hutton Melanie L ML   Abud Helen E HE   Peleg Anton Y AY   Lyras Dena D  

Journal of medical microbiology 20200131 2


<b>Introduction.</b> <i>Staphylococcus aureus</i> is a recognised cause of foodborne intoxication and antibiotic-associated diarrhoea (AAD), which are both mediated by staphylococcal enterotoxins. However, unlike foodborne intoxication, AAD appears to require infection of the host. While <i>S. aureus</i> intoxication is widely studied, little is known about <i>S. aureus</i> pathogenesis in the context of gastrointestinal infection.<b>Aim.</b> To develop a mouse model of <i>S. aureus</i> gastroin  ...[more]

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