Ontology highlight
ABSTRACT:
SUBMITTER: Djeghloul D
PROVIDER: S-EPMC7431861 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Djeghloul Dounia D Patel Bhavik B Kramer Holger H Dimond Andrew A Whilding Chad C Brown Karen K Kohler Anne-Céline AC Feytout Amelie A Veland Nicolas N Elliott James J Bharat Tanmay A M TAM Tarafder Abul K AK Löwe Jan J Ng Bee L BL Guo Ya Y Guy Jacky J Huseyin Miles K MK Klose Robert J RJ Merkenschlager Matthias M Fisher Amanda G AG
Nature communications 20200817 1
Epigenetic information is transmitted from mother to daughter cells through mitosis. Here, to identify factors that might play a role in conveying epigenetic memory through cell division, we report on the isolation of unfixed, native chromosomes from metaphase-arrested cells using flow cytometry and perform LC-MS/MS to identify chromosome-bound proteins. A quantitative proteomic comparison between metaphase-arrested cell lysates and chromosome-sorted samples reveals a cohort of proteins that wer ...[more]