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Transcriptional Inhibition of Sp-IAG by Crustacean Female Sex Hormone in the Mud Crab, Scylla paramamosain.


ABSTRACT: In crustaceans, the regulation of sex differentiation is mediated by insulin-like androgenic hormone (IAG) and crustacean female sex hormone (CFSH). CFSH is reported to inhibit IAG gene (Sp-IAG) expression in the mud crab Scylla paramamosain, but the regulatory mechanism is not well understood. A 2674 bp 5' flanking Sp-IAG contains many potential transcription factor binding sites. In this study, analysis of serially deleted 5' flanking Sp-IAG and site-directed mutation (SDM) of transcription factor binding sites of the same gene showed that the promoter activity of reporter vectors with Sox-5-binding site, signal transducers and activators of transcription (STAT)-binding site and activator protein 1 (AP-1)-binding site were significantly higher than that of vectors without these regions, suggesting that they were involved in transcriptional regulation of Sp-IAG expression. The expression analysis of these transcription factor showed that there was no difference in the level of mRNA in Sox-5 and AP-1 in androgenic gland treated with recombinant CFSH, but expression of Sp-STAT was significantly reduced, suggesting that CFSH regulates the expression of Sp-STAT, inhibiting its function to regulate Sp-IAG. Further experiment revealed that RNAi mediated Sp-STAT gene knockdown reduced the expression of Sp-IAG. These results suggested that Sp-CFSH regulates Sp-IAG by inhibiting STAT. This is a pioneering finding on the transcriptional mechanism of IAG gene in crustaceans.

SUBMITTER: Jiang Q 

PROVIDER: S-EPMC7432471 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Transcriptional Inhibition of <i>Sp-IAG</i> by Crustacean Female Sex Hormone in the Mud Crab, <i>Scylla paramamosain</i>.

Jiang Qingling Q   Lu Bei B   Wang Guizhong G   Ye Haihui H  

International journal of molecular sciences 20200726 15


In crustaceans, the regulation of sex differentiation is mediated by insulin-like androgenic hormone (IAG) and crustacean female sex hormone (CFSH). CFSH is reported to inhibit IAG gene (<i>Sp-IAG</i>) expression in the mud crab <i>Scylla paramamosain</i>, but the regulatory mechanism is not well understood. A 2674 bp 5' flanking <i>Sp-IAG</i> contains many potential transcription factor binding sites. In this study, analysis of serially deleted 5' flanking <i>Sp-IAG</i> and site-directed mutati  ...[more]

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