Double Bond Characterization of Free Fatty Acids Directly from Biological Tissues by Ultraviolet Photodissociation.

ABSTRACT: Free fatty acids (FA) are a vital component of cells and are critical to cellular structure and function, so much so that alterations in FA are often associated with cell malfunction and disease. Analysis of FA from biological samples can be achieved by mass spectrometry (MS), but these analyses are often not capable of distinguishing the fine structural alterations within FA isomers and often limited to global profiling of lipids without spatial resolution. Here, we present the use of ultraviolet photodissociation (UVPD) for the characterization of double bond positional isomers of charge inverted dication·FA complexes and the subsequent implementation of this method for online desorption electrospray ionization (DESI) MS imaging of FA isomers from human tissue sections. This method allow

SUBMITTER: Feider CL 

PROVIDER: S-EPMC7433749 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature