Dataset Information

In Vitro, in Vivo, and Spectroscopic Assessment of Lead Exposure Reduction via Ingestion and Inhalation Pathways Using Phosphate and Iron Amendments.

ABSTRACT: This study compared lead (Pb) immobilization efficacies in mining/smelting impacted soil using phosphate and iron amendments via ingestion and inhalation pathways using in vitro and in vivo assays, in conjunction with investigating the dynamics of dust particles in the lungs and gastro-intestinal tract via X-ray fluorescence (XRF) microscopy. Phosphate amendments [phosphoric acid (PA), hydroxyapatite, monoammonium phosphate (MAP), triple super phosphate (TSP), and bone meal biochar] and hematite were applied at a molar ratio of Pb:Fe/P = 1:5. Pb phosphate formation was investigated in the soil/post-in vitro bioaccessibility (IVBA) residuals and in mouse lung via extended X-ray absorption fine structure (EXAFS) and X-ray absorption near edge structures (XANES) spectroscopy, respectively. EXAFS analysis revealed that anglesite was the dominant phase in the ingestible (<250 ?m) and inhalable (<10 ?m) particle fractions. Pb IVBA was significantly reduced (p < 0.05) by phosphate amendments in the <250 ?m fraction (solubility bioaccessibility research consortium assay) and by PA, MAP, and TSP in the <10 ?m fraction (inhalation-ingestion bioaccessibility assay). A 21.1% reduction in Pb RBA (<250 ?m fraction) and 56.4% reduction in blood Pb concentration (<10 ?m fraction) were observed via the ingestion and inhalation pathways, respectively. XRF microscopy detected Pb in the stomach within 4 h, presumably via mucociliary clearance.

SUBMITTER: Kastury F

PROVIDER: S-EPMC7436645 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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In Vitro, in Vivo, and Spectroscopic Assessment of Lead Exposure Reduction via Ingestion and Inhalation Pathways Using Phosphate and Iron Amendments.

Kastury Farzana F Smith Euan E Doelsch Emmanuel E Lombi Enzo E Donnelley Martin M Cmielewski Patricia L PL Parsons David W DW Scheckel Kirk G KG Paterson David D de Jonge Martin D MD Herde Carina C Juhasz Albert L AL

Environmental science & technology 20190813 17

This study compared lead (Pb) immobilization efficacies in mining/smelting impacted soil using phosphate and iron amendments via ingestion and inhalation pathways using in vitro and in vivo assays, in conjunction with investigating the dynamics of dust particles in the lungs and gastro-intestinal tract via X-ray fluorescence (XRF) microscopy. Phosphate amendments [phosphoric acid (PA), hydroxyapatite, monoammonium phosphate (MAP), triple super phosphate (TSP), and bone meal biochar ...[more]

PMID: 31356748

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Dataset Information

In Vitro, in Vivo, and Spectroscopic Assessment of Lead Exposure Reduction via Ingestion and Inhalation Pathways Using Phosphate and Iron Amendments.

Publications

<i>In Vitro</i>, <i>in Vivo,</i> and Spectroscopic Assessment of Lead Exposure Reduction via Ingestion and Inhalation Pathways Using Phosphate and Iron Amendments.

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