Dataset Information

A new diagnostic strategy which uses a luminol-H2O2 system to detect helminth eggs in fecal sediments processed by the Helmintex method.

ABSTRACT: Schistosomiasis remains a serious public health problem in tropical regions, affecting more than 250 million people. Sensitive diagnostic methods represent key tools for disease elimination, in particular in areas with low endemicity. Advances in the use of luminol-based chemiluminescent techniques have enabled greater sensitivity and speed in obtaining results in different diagnostic settings. In this study, we developed a luminol-H2O2 chemiluminescence (CL) method to detect Schistosoma mansoni eggs in human fecal sediments processed by the Helmintex (HTX) method. After S. mansoni eggs were incubated with a solution of luminol-H2O2 the light emission was detected and measured by spectrophotometry at 431 nm for 5 min, using detection and counts of eggs by bright field optical microscopy as a reference. CL intensity was found to correlate with different sources and numbers of eggs. Furthermore, our results showed that the CL method can distinguish positive from negative samples with 100% sensitivity and 71% specificity. To our knowledge, this is the first study to report the use of CL for the diagnosis of helminths from fecal samples. The combination of the HTX method with CL represents an important advance in providing a reference method with the highest standards of sensitivity.

SUBMITTER: Favero V

PROVIDER: S-EPMC7437924 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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A new diagnostic strategy which uses a luminol-H2O2 system to detect helminth eggs in fecal sediments processed by the Helmintex method.

Favero Vivian V Veríssimo Carolina De Marco CM Piovesan Angela R AR Morassutti Alessandra L AL Souto André A AA Bittencourt Hélio R HR Pascoal Vanessa F VF Lindholz Catieli G CG Jones Malcolm K MK Souza Renata P RP Rigo Francine De Vargas FV Carlini Célia R CR Graeff-Teixeira Carlos C

PLoS neglected tropical diseases 20200730 7

Schistosomiasis remains a serious public health problem in tropical regions, affecting more than 250 million people. Sensitive diagnostic methods represent key tools for disease elimination, in particular in areas with low endemicity. Advances in the use of luminol-based chemiluminescent techniques have enabled greater sensitivity and speed in obtaining results in different diagnostic settings. In this study, we developed a luminol-H2O2 chemiluminescence (CL) method to detect Schistosoma mansoni ...[more]

PMID: 32730339

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Project description:Parasitic helminths, despite their known negative impact (biomaterial) on human health and animal production, have fascinating features. In this study, we find fantastic magnetic properties in several forms: inductor [between 20.10 and 58.85 (±2.50) H], source of detectable electrical voltage [from +0.5 to 7.3 (±0.1) V, vs. the ground, GND, measured by an AVO meter] and different inductor magnitude [between 3.33 and 41.23 (±0.76)] μH, detected by electrochemical impedance spectroscopy as well as frequency scannable electromagnetic wave horn) in several frequencies (including 100, 120, Hz, and 1, 10, 100 kHz) in "Fasciola hepatica", "Parascaris equorum" (with and without larvae), "Dicrocoelium dendriticum," "Taenia multiceps", and "Moniezia expansa" eggs. This claim is attributed to some surprising characteristics, including superior inductance and intrinsic magnetic susceptibility. This feature along with a close relationship to helminth egg structure, is a novel probe with acceptable reproducibility (RSD > 8.0%) and high enough trustworthiness for adequate differentiation in their magnitudes, relatively. These traits were measured by the "Single Cell Rrecording" methodology using a three-microelectrode system, implanted to each egg at the Giga ohm sealed condition (6.08 ± 0.22 GΩ cm-1, n = 5). The reliability of these results was further confirmed using multiple calibrated instruments such as a high-resolution inductance analyzer, LCR meter, impedance spectrometer, potentiometer, and an anomalous Hall effect (Magnetic field density) sensor. In addition, the critical role played (Synergistic Effect) by water-like molecules as the intermediate medium, besides the partial influence of other compounds such as dissolved oxygen, are investigated qualitatively, and specific relation between these molecules and magnetic field creation in helminth eggs was proved. These intrinsic characteristics would provide novel facilitators for efficient arriving at the researchable bio-based magnetic biomaterials, besides innovative and real-time identification probes in the "Parasitology" fields.

Dataset Information

A new diagnostic strategy which uses a luminol-H2O2 system to detect helminth eggs in fecal sediments processed by the Helmintex method.

Publications

A new diagnostic strategy which uses a luminol-H2O2 system to detect helminth eggs in fecal sediments processed by the Helmintex method.

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