Ontology highlight
ABSTRACT:
SUBMITTER: Hettiaratchi MH
PROVIDER: S-EPMC7438101 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Hettiaratchi Marian H MH O'Meara Matthew J MJ O'Meara Teresa R TR Pickering Andrew J AJ Letko-Khait Nitzan N Shoichet Molly S MS
Science advances 20200819 34
Maintaining biocatalyst stability and activity is a critical challenge. Chondroitinase ABC (ChABC) has shown promise in central nervous system (CNS) regeneration, yet its therapeutic utility is severely limited by instability. We computationally reengineered ChABC by introducing 37, 55, and 92 amino acid changes using consensus design and forcefield-based optimization. All mutants were more stable than wild-type ChABC with increased aggregation temperatures between 4° and 8°C. Only ChABC with 37 ...[more]