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Chemogenetic control of gene expression and cell signaling with antiviral drugs.

ABSTRACT: We developed a method in which the NS3 cis-protease from hepatitis C virus can be used as a ligand-inducible connection to control the function and localization of engineered proteins in mammalian cells. To demonstrate the versatility of this approach, we designed drug-sensitive transcription factors and transmembrane signaling proteins, the activities of which can be tightly and reversibly controlled through the use of clinically tested antiviral protease inhibitors.

SUBMITTER: Tague EP

PROVIDER: S-EPMC7439232 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Chemogenetic control of gene expression and cell signaling with antiviral drugs.

Tague Elliot P EP Dotson Hannah L HL Tunney Shannon N SN Sloas D Christopher DC Ngo John T JT

Nature methods 20180702 7

We developed a method in which the NS3 cis-protease from hepatitis C virus can be used as a ligand-inducible connection to control the function and localization of engineered proteins in mammalian cells. To demonstrate the versatility of this approach, we designed drug-sensitive transcription factors and transmembrane signaling proteins, the activities of which can be tightly and reversibly controlled through the use of clinically tested antiviral protease inhibitors. ...[more]

PMID: 29967495

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