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Active microrheology of a bulk metallic glass.

ABSTRACT: The glass transition remains unclarified in condensed matter physics. Investigating the mechanical properties of glass is challenging because any global deformation that might result in shear rejuvenation would require a prohibitively long relaxation time. Moreover, glass is well known to be heterogeneous, and a global perturbation would prevent exploration of local mechanical/transport properties. However, investigation based on a local probe, i.e., microrheology, may overcome these problems. Here, we establish active microrheology of a bulk metallic glass, via a probe particle driven into host medium glass. This technique is amenable to experimental investigations via nanoindentation tests. We provide distinct evidence of a strong relationship between the microscopic dynamics of the probe particle and the macroscopic properties of the host medium glass. These findings establish active microrheology as a promising technique for investigating the local properties of bulk metallic glass.

SUBMITTER: Yu JW

PROVIDER: S-EPMC7439307 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Active microrheology of a bulk metallic glass.

Yu Ji Woong JW Rahbari S H E SHE Kawasaki Takeshi T Park Hyunggyu H Lee Won Bo WB

Science advances 20200717 29

The glass transition remains unclarified in condensed matter physics. Investigating the mechanical properties of glass is challenging because any global deformation that might result in shear rejuvenation would require a prohibitively long relaxation time. Moreover, glass is well known to be heterogeneous, and a global perturbation would prevent exploration of local mechanical/transport properties. However, investigation based on a local probe, i.e., microrheology, may overcome these problems. H ...[more]

PMID: 32832632

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Project description:Polarization of macrophages by chemical, topographical and mechanical cues presents a robust strategy for designing immunomodulatory biomaterials. Here, we studied the ability of nanopatterned bulk metallic glasses (BMGs), a new class of metallic biomaterials, to modulate murine macrophage polarization. Cytokine/chemokine analysis of IL-4 or IFNγ/LPS-stimulated macrophages showed that the secretion of TNF-α, IL-1α, IL-12, CCL-2 and CXCL1 was significantly reduced after 24-hour culture on BMGs with 55 nm nanorod arrays (BMG-55). Additionally, under these conditions, macrophages increased phagocytic potential and exhibited decreased cell area with multiple actin protrusions. These in vitro findings suggest that nanopatterning can modulate biochemical cues such as IFNγ/LPS. In vivo evaluation of the subcutaneous host response at 2 weeks demonstrated that the ratio of Arg-1 to iNOS increased in macrophages adjacent to BMG-55 implants, suggesting modulation of polarization. In addition, macrophage fusion and fibrous capsule thickness decreased and the number and size of blood vessels increased, which is consistent with changes in macrophage responses. Our study demonstrates that nanopatterning of BMG implants is a promising technique to selectively polarize macrophages to modulate the immune response, and also presents an effective tool to study mechanisms of macrophage polarization and function. STATEMENT OF SIGNIFICANCE:Implanted biomaterials elicit a complex series of tissue and cellular responses, termed the foreign body response (FBR), that can be influenced by the polarization state of macrophages. Surface topography can influence polarization, which is broadly characterized as either inflammatory or repair-like. The latter has been linked to improved outcomes of the FBR. However, the impact of topography on macrophage polarization is not fully understood, in part, due to a lack of high moduli biomaterials that can be reproducibly processed at the nanoscale. Here, we studied macrophage interactions with nanopatterned bulk metallic glasses (BMGs), a class of metallic alloys with amorphous microstructure and formability like polymers. We show that nanopatterned BMGs modulate macrophage polarization and transiently induce less fibrotic and more angiogenic responses. Overall, we demonstrate nanopatterning of BMG implants as a technique to polarize macrophages and modulate the FBR.

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Active microrheology of a bulk metallic glass.

Publications

Active microrheology of a bulk metallic glass.

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