Ontology highlight
ABSTRACT:
SUBMITTER: Wang F
PROVIDER: S-EPMC7441100 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Wang Fei F Wang Yajuan Y Wan Zhimin Z Shao Hongxia H Qian Kun K Ye Jianqiang J Qin Aijian A
AMB Express 20200820 1
We previously reported a monoclonal antibody (mAb), 1G8, against the neuraminidase (NA) of H9N2 avian influenza virus (AIV) with significant NA inhibitory activity. To generate a recombinant chickenized mAb (RCmAb) against the NA of H9N2 AIV for passive immunization in poultry, the gene of the fragment of antigen binding (Fab) of mAb 1G8 was cloned and fused with the fragment crystallizable (Fc) gene of chicken IgY. The RCmAb 1G8 was expressed in COS-1 cells and could be detected in cell culture ...[more]