Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans.

ABSTRACT: Co-immunoprecipitation methods are frequently used to study protein-protein interactions. Confirmation of hypothesized protein-protein interactions or identification of new ones can provide invaluable information about the function of a protein of interest. Some of the traditional methods for extract preparation frequently require labor-intensive and time-consuming techniques. Here, a modified extract preparation protocol using a bead mill homogenizer and metal beads is described as a rapid alternative to traditional protein preparation methods. This extract preparation method is compatible with downstream co-immunoprecipitation studies. As an example, the method was used to successfully co-immunoprecipitate C. elegans microRNA Argonaute ALG-1 and two known ALG-1 interactors: AIN-1, and HR

SUBMITTER: Li L 

PROVIDER: S-EPMC7441831 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature