Ontology highlight
ABSTRACT:
SUBMITTER: Suzuki-Hatano S
PROVIDER: S-EPMC7442215 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
Suzuki-Hatano Silveli S Tsai Ang-Chen AC Daugherty Audrey A Pacak Christina A CA
Journal of visualized experiments : JoVE 20200608 160
Proteomic technologies are powerful methodologies that can aid our understanding of mechanisms of action in biological systems by providing a global view of the impact of a disease, treatment, or other condition on the proteome as a whole. This report provides a detailed protocol for the extraction, quantification, precipitation, digestion, labeling, and subsequent data analysis of protein samples. Our optimized TMT labeling protocol requires a lower tag-label concentration and achieves consiste ...[more]