Dataset Information

Prognostic Significance of Immune Cell Populations Identified by Machine Learning in Colorectal Cancer Using Routine Hematoxylin and Eosin-Stained Sections.

ABSTRACT: PURPOSE:Although high T-cell density is a well-established favorable prognostic factor in colorectal cancer, the prognostic significance of tumor-associated plasma cells, neutrophils, and eosinophils is less well-defined. EXPERIMENTAL DESIGN:We computationally processed digital images of hematoxylin and eosin (H&E)-stained sections to identify lymphocytes, plasma cells, neutrophils, and eosinophils in tumor intraepithelial and stromal areas of 934 colorectal cancers in two prospective cohort studies. Multivariable Cox proportional hazards regression was used to compute mortality HR according to cell density quartiles. The spatial patterns of immune cell infiltration were studied using the GTumor:Immune cell function, which estimates the likelihood of any tumor cell in a sample having at least one neighboring immune cell of the specified type within a certain radius. Validation studies were performed on an independent cohort of 570 colorectal cancers. RESULTS:Immune cell densities measured by the automated classifier demonstrated high correlation with densities both from manual counts and those obtained from an independently trained automated classifier (Spearman's ? 0.71-0.96). High densities of stromal lymphocytes and eosinophils were associated with better cancer-specific survival [P trend < 0.001; multivariable HR (4th vs 1st quartile of eosinophils), 0.49; 95% confidence interval, 0.34-0.71]. High GTumor:Lymphocyte area under the curve (AUC0,20?m; P trend = 0.002) and high GTumor:Eosinophil AUC0,20?m (P trend < 0.001) also showed associations with better cancer-specific survival. High stromal eosinophil density was also associated with better cancer-specific survival in the validation cohort (P trend < 0.001). CONCLUSIONS:These findings highlight the potential for machine learning assessment of H&E-stained sections to provide robust, quantitative tumor-immune biomarkers for precision medicine.

SUBMITTER: Vayrynen JP

PROVIDER: S-EPMC7442724 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Prognostic Significance of Immune Cell Populations Identified by Machine Learning in Colorectal Cancer Using Routine Hematoxylin and Eosin-Stained Sections.

Väyrynen Juha P JP Lau Mai Chan MC Haruki Koichiro K Väyrynen Sara A SA Dias Costa Andressa A Borowsky Jennifer J Zhao Melissa M Fujiyoshi Kenji K Arima Kota K Twombly Tyler S TS Kishikawa Junko J Gu Simeng S Aminmozaffari Saina S Shi Shanshan S Baba Yoshifumi Y Akimoto Naohiko N Ugai Tomotaka T Da Silva Annacarolina A Song Mingyang M Wu Kana K Chan Andrew T AT Nishihara Reiko R Fuchs Charles S CS Meyerhardt Jeffrey A JA Giannakis Marios M Ogino Shuji S Nowak Jonathan A JA

Clinical cancer research : an official journal of the American Association for Cancer Research 20200521 16

<h4>Purpose</h4>Although high T-cell density is a well-established favorable prognostic factor in colorectal cancer, the prognostic significance of tumor-associated plasma cells, neutrophils, and eosinophils is less well-defined.<h4>Experimental design</h4>We computationally processed digital images of hematoxylin and eosin (H&E)-stained sections to identify lymphocytes, plasma cells, neutrophils, and eosinophils in tumor intraepithelial and stromal areas of 934 colorectal cancers in two prospec ...[more]

PMID: 32439699

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Dataset Information

Prognostic Significance of Immune Cell Populations Identified by Machine Learning in Colorectal Cancer Using Routine Hematoxylin and Eosin-Stained Sections.

Publications

Prognostic Significance of Immune Cell Populations Identified by Machine Learning in Colorectal Cancer Using Routine Hematoxylin and Eosin-Stained Sections.

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