Continuous bioactivity-dependent evolution of an antibiotic biosynthetic pathway.
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ABSTRACT:
SUBMITTER: Johnston CW
PROVIDER: S-EPMC7443133 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Publications
Continuous bioactivity-dependent evolution of an antibiotic biosynthetic pathway.
Johnston Chad W CW Badran Ahmed H AH Collins James J JJ
Nature communications 20200821 1
Antibiotic biosynthetic gene clusters (BGCs) produce bioactive metabolites that impart a fitness advantage to their producer, providing a mechanism for natural selection. This selection drives antibiotic evolution and adapts BGCs for expression in different organisms, potentially providing clues to improve heterologous expression of antibiotics. Here, we use phage-assisted continuous evolution (PACE) to achieve bioactivity-dependent adaptation of the BGC for the antibiotic bicyclomycin (BCM), fa ...[more]
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