Rate zonal centrifugation can partially separate platelets from platelet-derived vesicles.

ABSTRACT:

Background

Centrifugation is commonly used as a first step to enrich biomarkers from blood. Biomarkers are separated on the basis of density and/or diameter. However, the centrifugation protocol affects the yield and purity of biomarkers, for example, isolation of platelets results in co-isolation with extracellular vesicles (EVs).

Objective

To assess the ability of rate zonal centrifugation (RZC) to separate platelets from co-isolated EVs.

Methods

Using a linear Optiprep gradient, RZC was able to separate a mixture of beads with different diameters but similar density. Next, RZC was applied to samples containing both platelets and platelet-derived EVs (n = 3). After RZC, all fractions were collected and stained with anti-CD61-Alexa 488 to measure the concentrations o

SUBMITTER: Rikkert LG 

PROVIDER: S-EPMC7443435 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature