Direct imaging of liquid domains in membranes by cryo-electron tomography.

ABSTRACT: Images of micrometer-scale domains in lipid bilayers have provided the gold standard of model-free evidence to understand the domains' shapes, sizes, and distributions. Corresponding techniques to directly and quantitatively assess smaller (nanoscale and submicron) liquid domains have been limited. Researchers commonly seek to correlate activities of membrane proteins with attributes of the domains in which they reside; doing so hinges on identification and characterization of membrane domains. Although some features of membrane domains can be probed by indirect methods, these methods are often constrained by the limitation that data must be analyzed in the context of models that require multiple assumptions or parameters. Here, we address this challenge by developing and testing two metho

SUBMITTER: Cornell CE 

PROVIDER: S-EPMC7443872 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature