Third-generation sequencing found LncRNA associated with heat shock protein response to heat stress in Populus qiongdaoensis seedlings.

ABSTRACT: BACKGROUND:As air temperatures increase globally, more and more plants are exposed to heat-stress conditions. Although many studies have explored regulation networks in plants with the aim of improving their heat-stress tolerance, only few have revealed them in trees. Here, individuals of Populus qiongdaoensis seedlings, which grows naturally in tropical areas, exposed to heat at 40?°C and the non-coding regulation networks were explored using the PacBio RSII and the Illumina sequencing platform. RESULTS:In total, we obtained 88,161 full-length transcripts representing 39,343 genes using 5,498,988 long reads and 350,026,252 clean reads, and also 216 microRNAs (miRNAs) via 95,794,107 reads. We then identified 928 putative long non-coding RNAs (lncRNAs), consisting of 828 sense lncRNAs (89.22%), 34 long intergenic non-coding RNAs (3.66%), 16 antisense (1.72%), and 50 sense intronic lncRNAs (5.39%). Under the dual criteria of |log2fold-change|???1 and P-value

SUBMITTER: Xu J 

PROVIDER: S-EPMC7444055 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature