Dataset Information

Engineering luminescent biosensors for point-of-care SARS-CoV-2 antibody detection.

ABSTRACT: Current serology tests for SARS-CoV-2 antibodies mainly take the form of enzyme-linked immunosorbent assays or lateral flow assays, with the former being laborious and the latter being expensive and often lacking sufficient sensitivity and scalability. Here we present the development and validation of a rapid, low-cost solution-based assay to detect antibodies in serum, plasma, whole blood, and saliva, using rationally designed split luciferase antibody biosensors (spLUC). This new assay, which generates quantitative results in as short as 5 minutes, substantially reduces the complexity and improves the scalability of COVID-19 antibody tests for point-of-care and broad population testing.

SUBMITTER: Elledge SK

PROVIDER: S-EPMC7444307 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Engineering luminescent biosensors for point-of-care SARS-CoV-2 antibody detection.

Elledge Susanna K SK Zhou Xin X XX Byrnes James R JR Martinko Alexander J AJ Lui Irene I Pance Katarina K Lim Shion A SA Glasgow Jeff E JE Glasgow Anum A AA Turcios Keirstinne K Iyer Nikita N Torres Leonel L Peluso Michael J MJ Henrich Timothy J TJ Wang Taia T TT Tato Cristina M CM Leung Kevin K KK Greenhouse Bryan B Wells James A JA

medRxiv : the preprint server for health sciences 20200821

Current serology tests for SARS-CoV-2 antibodies mainly take the form of enzyme-linked immunosorbent assays or lateral flow assays, with the former being laborious and the latter being expensive and often lacking sufficient sensitivity and scalability. Here we present the development and validation of a rapid, low-cost solution-based assay to detect antibodies in serum, plasma, whole blood, and saliva, using rationally designed split luciferase antibody biosensors (spLUC). This new assay, which ...[more]

PMID: 32839788

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Project description:Antibody detection assays are essential for evaluating immunity of individuals against a given virus, and this has been particularly relevant during the COVID-19 pandemic. Current serology assays either require a laboratory setting and take >1 hr (i.e., enzyme-linked immunosorbent assay [ELISA]) or are rapid but only qualitative in nature and cannot accurately track antibody levels over time (i.e., lateral flow assay [LFA]). Therefore, there is a need for development of a rapid and simple but also quantitative assay that can evaluate antibody levels in patients accurately over time. We have developed an assay that uses a split nanoluciferase fused to the spike or nucleocapsid proteins of the SARS-CoV-2 virus to enable luminescent-based detection of spike- or nucleocapsid-binding antibodies in serum, plasma, and whole blood samples. The resulting approach is simple, rapid, and quantitative and is highly amenable to low-/medium-throughput scale using plate-based assays, high-throughput scale using robotics, and point-of-care applications. In this article, we describe how to perform the assay in a laboratory setting using a plate reader or liquid-handling robotics and in a point-of-care setting using a handheld, battery-powered luminometer. Together, these assays allow antibody detection to be easily performed in multiple settings by simplifying and reducing assay time in a laboratory or clinical environment and by allowing for antibody detection in point-of-care, nonlaboratory settings. © 2022 Wiley Periodicals LLC. Basic Protocol: SARS-CoV-2 antibody detection using the split-luciferase assay on a medium-throughput scale with a laboratory luminometer Alternate Protocol 1: High-throughput-based protocol for SARS-CoV-2 antibody detection using a robotic platform Alternate Protocol 2: Point-of-care-based protocol for SARS-CoV-2 antibody detection using a handheld luminometer Support Protocol: Determining positive/negative cutoffs for test samples and standardizing the assay between days.

Dataset Information

Engineering luminescent biosensors for point-of-care SARS-CoV-2 antibody detection.

Publications

Engineering luminescent biosensors for point-of-care SARS-CoV-2 antibody detection.

Similar Datasets

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