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Transcriptional and Functional Analysis of CD1c⁺ Human Dendritic Cells Identifies a CD163⁺ Subset Priming CD8⁺CD103⁺ T Cells.

ABSTRACT: Dendritic cells (DCs) are antigen-presenting cells controlling T cell activation. In humans, the diversity, ontogeny, and functional capabilities of DC subsets are not fully understood. Here, we identified circulating CD88^-CD1c⁺CD163⁺ DCs (called DC3s) as immediate precursors of inflammatory CD88^-CD14⁺CD1c⁺CD163⁺FcεRI⁺ DCs. DC3s develop via a specific pathway activated by GM-CSF, independent of cDC-restricted (CDP) and monocyte-restricted (cMoP) progenitors. Like classical DCs but unlike monocytes, DC3s drove activation of naive T cells. In vitro, DC3s displayed a distinctive ability to prime CD8⁺ T cells expressing a tissue homing signature and the epithelial homing alpha-E integrin (CD103) through transforming growth factor β (TGF-β) signaling. In vivo, DC3s infiltrated luminal breast cancer primary tumors, and DC3 infiltration correlated positively with CD8⁺CD103⁺CD69⁺ tissue-resident memory T cells. Together, these findings define DC3s as a lineage of inflammatory DCs endowed with a strong potential to regulate tumor immunity.

SUBMITTER: Bourdely P

PROVIDER: S-EPMC7445430 | biostudies-literature |

REPOSITORIES: biostudies-literature

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